This study investigates entrepreneurship education ecosystems in public research universities in Southeast Asia, focusing on the cases of one public university in Singapore and one public university in Malaysia. It explores how curricula and practical experiences influence students' entrepreneurial motivation and outcomes, identifying gaps and strengths in current educational frameworks. Using a qualitative research design, data were collected through semi-structured interviews with ten master's students enrolled in entrepreneurship courses, all possessing business management backgrounds and at least one year of work or entrepreneurial experience. Thematic analysis revealed that forward-looking, industry-aligned curricula stimulate entrepreneurial interest, while outdated, theory-heavy content hinders application. Practical experiences, such as internships and workshops, significantly enhance entrepreneurial skills and confidence, though limited opportunities remain a challenge. This study contributes to the theoretical understanding of the link between entrepreneurship education and motivation, highlighting the importance of practical integration. It offers recommendations for universities to update curricula, enhance industry collaborations, and expand practical opportunities to better prepare students for entrepreneurial ventures.